UNITED STATES DEPARTMENT OF AGRICULTURE
COOPERATIVE STATE RESEARCH, EDUCATION, AND EXTENSION SERVICE

OMB Approved 0524-0024
Expires 08/98

Give the following information for each key professional associated with the project, beginning with the Project Director. Do not exceed the three-page form. If necessary, provide selected examples deemed most appropriate for purposes of this grant application. Photocopy additional forms as needed.

Education (Begin with baccalaureate or other initial professional education and include postdoctoral training)

2. Professional Experience (Beginning with present position, list employment by title, affiliation, and dates in last 10 years)

1968- University Distinguished Professor, Departments of Crop and Soil Science and Microbiology and Public Health, Michigan State University

1989- Director, Center for Microbial Ecology, an NSF National Science and Technology Center

3. Teaching Experience (Cite titles and summary catalog descriptions of undergraduate and graduate level courses for which you have had primary responsibility for teaching in last 5 years)

MPH 425 (3 cr) Microbial Ecology - Microbial population and community interactions. Microbial activities in natural systems, including associations with plants or animals.

MPH 821 (3 cr) Microbial Physiology - Molecular architecture, assembly of cell parts, metabolism, and general physiology of typical eubacteria.

CSS 455 (3 cr) Pollutants in Soil Environment - Chemical and biological reacitons of organic and inorganic pollutants in soils.

4. Research Interests: Microbial ecology as it relates to denitrification, reductive dehalogenation, and the use of gene probes to study community selection in nature.

5. Thesis Committees Chaired (Cite titles and dates of Master's and doctoral theses completed or in progress by graduate students for whom you served as committee chair in last 5 years)

Ye, R.W., Ph.D., 1992, Dissimilatory Reduction of Nitrite and Nitric Oxide by Denitrifying Bacteria.

Ka, J.O., Ph.D., 1993, a Molecular Approach to Understanding the Genetic Response of 2,4-D Degrading Microbial Populations in Soils.

Santo Domingo, J., Ph.D., 1994, Use of Nucleic Acid Based Methods to Study the Bacterial Community of the Cricket Hindgut.

Rhodes, A., Ph.D., 1994, Distribution and Catabolic Diversity of 3-Chlorobenzoic Acid Degrading Bacteria Isolated from Geographically-Separated Pristine Soils.

Massol-Deyá, A., Ph.D., 1994, Microbial Biofilm Communities in Granular Activated Carbon Fluidized Bed Reactors for Treatment of Groundwater Contaminated with Aromatic Hydrocarbons.

McGowan, C., Ph.D., 1995, Interspecies Gene Transfer in the Evolution of 2,4-Dichlorophenoxyacetate Degrading Bacteria.

Fries, M.R., Ph.D., 1995, Diversity of Mono Aromatic Hydrocarbon Degrading Bacteria and Their TCE Co-oxidation Potential.

Chee-Sanford, J.C., Ph.D., 1996, The Physiology and the Characteristics of the Upper Pathway of Anaerobic Toluene Degradation in a New Bacterium Azoarcus Tolulyticus Strain Tol-4.

Sanford, R.A., Ph.D., 1996, Characterization of Microbial Populations in Anaerobic Food Webs that Reductively Dechlorinate Chlorophenols.

Nuesslein, K., Ph.D., 1997, Structure of Pseudomonas Communities Along Age, Vegetation and Soil Gradients in Hawaii.

Rodrigues, J., Ph.D., 1999, Molecular Characterization of Genes Involved in Dechlorination Useful in PCB Degradation.

Asuming-Brempong, S., Ph.D. 1999, Use of Molecular and Process Methods to Determine Microbial Community Shifts due to Long-Term Pesticide Application.

Griffin, B., Ph.d., 2000, Elucidation of the Pathway of Anaerobic Toluene Degradation in Azoarcus, an Aquifer Denitrifier.

6. Grants (Cite title, amount, source, and period of support for research and teaching/instructional projects directed or co- directed in last 10 years)

Dr. Tiedje has directed or co-directed research and/or teaching/instructional/demonstration projects which received $20,838,556 in support over the last 10 years and a total of $24,771,914 since beginning his faculty appointment at Michigan State University in 1970. See Appendix 4 for more detailed information relative to individual projects, their sponsors and the level of financial support.

7. Professional Honors (Last 10 years)

Fellow, International Institute of Biotechnology (London), 1991

Applied and Environmental Research Award, American Society for Microbiology, 1992

University Distinguished Professor, 1992

Editor, Microbiological Reviews, 1992-1995

8. Publications (List in chronological order the titles and complete references for printed publications in last 5 years)

Dr. Tiedje has authored/co-authored 61 peer reviewed papers and 17 book chapters since 1990 in addition to the following publications:

Ka, J.O., W.E. Holben and J.M. Tiedje. 1994. Use of gene probes to aid in recovery and identification of functinally dominant 2,4-dichlorophenoxyacetic acid-degrading populations in soil. Appl. Environ. Microbiol. 60:1116-1120.

Ka, J.O., W.E. Holben and J.M. Tiedje. 1994. Analysis of competition in soil among 2,4-dichlorophenoxyacetic acid-degrading bacteria. Appl. Environ. Microbiol. 60:1121-1128.

Ka, J.O. and J.M. Tiedje. 1994. Integration and excision of a 2,4-dichlorophenoxyacedtic acid-degradative plasmid in Alcaligenes paradoxus and evidence of its natural intergeneric transfer. J. Bacteriol. 176:5284-5289.

Krumme, M.L., R.L. Smith, S.M. Thiem, J. Egestorff, J.M. Tiedje, K.N. Timmis and D.F. Dwyer. 1994. Behavior of pollutant-degrading organisms in aquifers; predictions for genetically engineered organisms. Environ. Sci. Technol. 28:1134-1138.

Tiedje, J.M., J.F. quensen, III, J. Chee-Sanford, J.P. Schimel and S.A. Boyd. 1994. Microbial reductive dechloriantion of PCBs. Biodegradation 4:231-240.

Fries, M.R., J.Z. Zhou, J.C. Chee-Sanford and J.M. Tiedje. 1994. Isolation, characterizationa nd distribution of denitrifying toluene degraders from a variety of habitats. Appl. Environ. Microbiol. 60:2802-2810.

Cole, J.R., A.L. Cascarelli, W.W. Mohn, and J.M. Tiedje. 1994. Isolation and characterization of a novel bacterium growing via reductive dehalogenation of 2-chlorophenol. Appl. Environ. Microbiol. 60:3536-3542.

Fulthorpe, R.R., A.N. Rhodes and J.M. Tiedje. 1996. Pristine soils mineralize 3-chlorobenzoate and 2,4-dichlorophenoxyacetate via different microbial populations. Appl. Microbiol. 62:1159-1166.

Pellizari, V.H., S. Bezborodnikov, J.F. Quensen, III, and J.M. Tiedje. 1996. Evaluation of strains isolated by growth on naphthalene and biphenyl for hybridization of genes to dioxygenase probes and polychlorinated biphenyl-degrading ability. Appl. Environ. Microbiol. 62:2053-2058.

Moyer, C.L., J.M. Tiedje, F.C. Dobbs and D.M. Karl. 1996. A computer-simulated restriction fragment length polymorphism analysis of bacterial small-subunit rRNA genes: efficacy of selected tetrameric restriction enzymes for studies of microbial diversity in nature. Appl. Environ. Microbiol. 62:2501-2507.

Shih, C.-C., M.E. Davey, J. Zhou, J.M. Tiedje and C.S. Criddle. 1996 Effects of phenol feeding pattern on microbial community structure and cometabolism of trichloroethylene. Appl. Environ. Microbiol. 62:2953-2960.

Sanford, R.A., J.R. Cole, F.E. Löffer and J.M. Tiedje. 1996. Characterization of Desulfitobacterium chloroespirans sp. nov., which grows by coupling the oxidation of lactate to the reductive dechlorination of 3-chloro-4-hydroxybenzoate. Appl. Environ. Microbiol. 62:3800-3808.

Löffler, F.E., R.A. Sanford and J.M. Tiedje. 1996. Initial characterization of a reductive dehalogenase from Desulfitobacterium chlororespirans strain Co23. Appl. Environ. Microbiol. 62:3809-3813.

Tiedje, J.M., J. Urbance, N. Larsen, t. Schmidt, O. Strunk, S. Pramanik, R. Overbeek, R. Martin and J. Holt. 1996. Towards an integrated microbial database. Pp. 63-68. In: Culture collections to improve the quality of life; R.A. Samson, J.A. Stalpers, D. van der Mei, and A.H. Stouthamer (eds.), CBS Publishing, Baarn, The Netherlands.

Tiedje, J.M. and J. Zhou. 1996. Analysis of non-culturable bacteria. pp. 53-65. In: Methods for the examination of organismal diversity in soils and sediments. Geoffrey S. Hall (ed)., CAB International.

Massol-Deyá, A., R. Weller, L. Ríos-Hernández, J.-Z. Zhou, R.F. Hickey, and J.M. Tiedje. 1997. Succession and convergence of biofilm communities in fixed-film reactors treating aromatic hydrocarbons in groundwater. Appl. Environ. Microbiol. 63:270-276.

Sanford, R.A. and J.M. Tiedje. 1997. Chlorophenol dechlorination and subsequent degradation in denitrifying microcosms fed low concentrations of nitrate. Biodegradation 7:425-434.

Munakata-Marr, J., V.G. Matheson, L.J. Forney, J.M. Tiedje and P.L. McCarty. 1997. Microbial succession during a field evaluation of phenol and toluene as the primary substrates for trichloroethene cometabolism. Appl. Environ. Microbiol. 63:1515-1522.

Fries, M.R., L.J. Forney and J.M. Tiedje. 1997. Phenol-and toluene-degrading microbial populations from an aquifer in which successful trichloroethene cometabolism occurred. Appl. Environ. Microbiol. 63:1523-1530.